Analysis Note

ControlCONTROL ANTIGEN: Included free of charge with the antibody is 40 µg of control antigen. The stock solution of the antigen can be made up using 100 µL of sterile distilled water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Western blot: 1:200-1:400 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Anti-HCN1 Antibody is an antibody against HCN1 for use in IH & WB.

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide corresponding amino acids 6-24 of rat HCN-1 (Accession number AAF62173).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Specificity

Recognizes HCN-1 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 1, HAC2, BCNG1). The epitope does not share homology with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also react with mouse (18/19) and possibly rabbit (16/19) due to sequence homology. Other species have not been tested.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.